Generator

Part:BBa_K215011:Design

Designed by: Jeff Nivala   Group: iGEM09_Washington   (2009-10-01)


K215090+K215002: OpdA with Secretion Signal and Streptavidin Binding Tags


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 84
    Illegal suffix found in sequence at 1982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 84
    Illegal SpeI site found at 1983
    Illegal PstI site found at 1997
    Illegal NotI site found at 90
    Illegal NotI site found at 1990
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 84
    Illegal XhoI site found at 1211
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 84
    Illegal suffix found in sequence at 1983
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 84
    Illegal XbaI site found at 99
    Illegal SpeI site found at 1983
    Illegal PstI site found at 1997
    Illegal NgoMIV site found at 308
    Illegal AgeI site found at 503
    Illegal AgeI site found at 842
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References